Novel dense CO2 technique for beta-galactosidase immobilization in polystyrene microchannels

作者：Ellis Jeffrey LeClair; Tomasko David L^*; Dehghani Fariba

来源：Biomacromolecules, 2008, 9(3): 1027-1034.

DOI：10.1021/bm701343m

In this study we design new fabrication techniques and demonstrate the potential of using dense CO2, for facilitating crucial steps in the fabrication of polymeric lab-on-a-chip microdevices by embedding biomolecules at temperatures well below the polymer's glass transition temperature (T-g). These new techniques are environmentally friendly and done without the use of a clean room. Carbon dioxide at 40 degrees C and between 4.48 and 6.89 MPa was used to immobilize the biologically active molecule, beta-galactosidase (beta-gal), on the surface of polystyrene microchannels. To our knowledge, this is the first time dense CO, has been used to directly immobilize an enzyme in a microchannel. beta-gal activity was maintained and shown via a fluorescent reaction product, after enzyme immobilization and microchannel capping by the designed fabrication steps at 40 degrees C and pressures up to 6.89 MPa.

• 出版日期2008-3