ERK-Mediated Phosphorylation of Fibroblast Growth Factor Receptor 1 on Ser(777) Inhibits Signaling

作者：Zakrzewska Malgorzata; Haugsten Ellen Margrethe; Nadratowska Wesolowska Beata; Oppelt Angela; Hausott Barbara; Jin Yixin; Otlewski Jacek; Wesche Jorgen; Wiedlocha Antoni^*

来源：Science Signaling, 2013, 6(262): ra11.

DOI：10.1126/scisignal.2003087

Fibroblast growth factor 1 (FGF1) controls cellular activities through the activation of specific cell-surface FGF receptors (FGFRs). Transphosphorylation of tyrosine residues in the kinase domain of FGFRs leads to activation of intracellular signaling cascades, including those mediated by mitogen-activated protein kinases (MAPKs). FGFRs also contain a serine-rich C-terminal tail. We identified a regulatory mechanism of FGFR signaling involving phosphorylation of Ser(777) in the C-terminal region of FGFR1 by the MAPKs extracellular signal-regulated kinase 1 (ERK1) and ERK2. Prevention of the phosphorylation of Ser(777) in FGFR1 or mutation of Ser(777) to alanine enhanced FGF-stimulated receptor tyrosine phosphorylation and increased cell proliferation, cell migration, and axonal growth. A form of FGFR1 with a phosphomimetic mutation at Ser(777) exhibited reduced signaling. Activation of MAPKs by other receptor tyrosine kinases also resulted in phosphorylation of Ser(777) in FGFR1, thereby enabling crosstalk regulation of FGFR activity by other signaling pathways. Our data reveal a negative feedback mechanism that controls FGF signaling and thereby protects the cell from excessive activation of FGFR.

• 出版日期2013-2-12