Acetylcholinesterase inhibitors from galbanum, the oleo gum-resin of Ferula gummosa Boiss.

作者：Adhami Hamid Reza^*; Fitz Veronika; Lubich Andrea; Kaehlig Hanspeter; Zehl Martin; Krenn Liselotte

来源：Phytochemistry Letters, 2014, 10: LXXXII-lxxxvii.

DOI：10.1016/j.phytol.2014.08.023

• Galbanum
• Acetylcholinesterase inhibition
• Kellerin
• Deacetylkellerin
• 5 %26apos
• -Hydroxyauraptene
• 5 %26apos
• -Acetoxyauraptene

Due to the correlation of Alzheimer%26apos;s disease with a cholinergic deficit, acetylcholinesterase (AChE) inhibitors from herbal drugs or herbal medicinal preparations have gained a lot of scientific interest. In a previous study, several substances with AChE inhibitory activity were detected in galbanum by TLC bioautography and two of them were identified as auraptene and farnesiferol A. The aim of present study was the isolation and characterization of the other active compounds in galbanum. In an activity-guided approach, six components were isolated via column chromatography and solid phase extraction. The compounds were identified as deacetylkellerin, kellerin, umbelliferone, 5%26apos;-acetoxyauraptene, 5%26apos;-hydroxyauraptene and farnesiferol B and their concentrations in galbanum were determined by HPLC analysis with external standardization. Kellerin showed the best AChE inhibition with an IC50 value of 10.6 mu M which was about one third of the positive control physostigmine (IC50 2.91 mu M).

• 出版日期2014-12