Analysis of the expression of N-cadherin and survivin in an established D-17 cell line and in canine cells of spontaneous osteosarcoma

作者：Ciaputa Rafal^*; Poradowski Dominik; Janus Izabela; Nowak Marcin; Obminska Mrukowicz Bozena; Madej Janusz A; Kandefer Gola Malgorzata

来源：Medycyna Weterynaryjna, 2015, 71(6): 349-353.

• immunohistochemistry
• N-cadherin
• survivin
• canine osteosarcoma

The aim of the study was to demonstrate and evaluate the expression of N-cadherin and survivin in spontaneous osteosarcoma tumours in dogs compared to the expression of these proteins in an established (D-17) cell line of canine osteosarcoma. Osteosarcoma samples were taken from the limbs of 15 dogs and fixed in 7% buffered formalin. An adherent canine osteosarcoma cell line (D-17) was also used for the study. The cytoplasmic expression of N-cadherin and survivin was shown in spontaneous osteosarcoma, whereas the cytoplasmic and nuclear expression of N-cadherin and survivin was observed in cells derived from the D-17 cell line. The results indicate that the higher the cytoplasmic expression of N-cadherin, the higher the expression of survivin. By analyzing the strength of intercellular adhesion, which is based on the type and strength of N-cadherin expression, as well as the degree of apoptosis inhibition, one may conclude that these markers may be used as supplementary to routine tests to evaluate the degree of the tumour's malignancy and the patient's prognosis.

• 出版日期2015-6