Mu transpososome and RecBCD nuclease collaborate in the repair of simple Mu insertions

作者：Choi Wonyoung; Jang Sooin; Harshey Rasika M^*

来源：Proceedings of the National Academy of Sciences, 2014, 111(39): 14112-14117.

DOI：10.1073/pnas.1407562111

• conservative integration
• nonreplicative transposition

The genome of transposable phage Mu is packaged as a linear segment, flanked by several hundred base pairs of non-Mu DNA. The linear ends are held together and protected from nucleases by the phage N protein. After transposition into the Escherichia coli chromosome, the flanking DNA (FD) is degraded, and the 5-bp gaps left in the target are repaired to generate a simple Mu insertion. Our study provides insights into this repair pathway. The data suggest that the first event in repair is removal of the FD by the RecBCD exonuclease, whose entry past the N-protein block is licensed by the transpososome. In vitro experiments reveal that, when RecBCD is allowed entry into the FD, it degrades this DNA until it arrives at the transpososome, which presents a barrier for further RecBCD movement. RecBCD action is required for stimulating endonucleolytic cleavage within the transpososome-protected DNA, leaving 4-nt flanks outside both Mu ends. This end product of collaboration between the transpososome and RecBCD resembles the intermediate products of Tn7 and retroviral and retro-transposon transposition, and may hint at a common gap-repair mechanism in these diverse transposons.

• 出版日期2014-9-30