科研之友
微信
新浪微博
Facebook
分享链接
摘要
An amperometric immunosensor for cardiac troponin T detection in human serum troponin T, a marker considered as "gold standard" for acute myocardial infarction diagnosis, is described. A stable carboxylic film to covalently bind antibodies against cTnT onto electrode surface was achieved with electropolymerization of the o-aminobenzoic acid. A fractional factorial study was performed to optimize the electropolymerization parameters. Cyclic voltammetry assays were carried out for characterize steps of the modified electrode surface. The obtained calibration curve at -0.05 V by amperometry presented a good linear response range from 0.05 to 5.0 ngmL(-1) cTnT with a correlation coefficient of 0.992 (n = 6) and 0.016 ng mL(-1) detection limit. The electrodes showed a good stability upon the analytical responses retaining 91.6% of its initial response after 18 days. This sensor showed outgoing results regarding sensitivity allowing reliable measurements of the cTnT at levels of clinical significance for acute myocardial infarctions diagnosis.
- 出版日期2013-2-15
