Toll-like receptor 3-mediated tumor invasion in head and neck cancer

作者：Chuang Hui Ching; Huang Chao Cheng; Chien Chih Yen; Chuang Jiin Haur^*

来源：Oral Oncology, 2012, 48(3): 226-232.

DOI：10.1016/j.oraloncology.2011.10.008

• CCL5
• Head and neck cancer
• IL-6
• Oral cancer
• Poly I:C
• Toll-like receptor 3

Objectives: Chronic inflammation associated with some infectious agents can lead to cancer. The Toll-like receptor (TLR) family is one of the largest and best-studied families of pathogen-associated molecular patterns. TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. %26lt;br%26gt;Materials and methods: We investigated TLR3 protein expression in 153 oral squamous cell carcinoma (OSCC) specimens using tissue microarray. Furthermore, we used polyinosinic-polycytidylic acid (poly I:C) to stimulate head and neck cancer cells and an inhibitor of endosomal acidification bafilomycin A1 to block the TLR 3 signaling pathway to clarify the role of TLR 3 in OSCC. %26lt;br%26gt;Results: Cytoplasmic TLR3 staining was observed in the vast majority of OSCC tissues (73.2%). Strong TLR3 expression was significantly correlated with patients whose tumors were poorly differentiated (P = 0.028) and with perineural invasion (P = 0.023). Three of the four head and neck cell lines tested (Fadu, OC2, and SCC4) expressed TLR3 mRNA, although at various levels. The stimulation of TLR3-expressing OC2 cells with poly I:C caused the phosphorylation of IFN regulatory factor 3 and I kappa B and sequentially induced the secretion of interleukin-6 and chemokine (C-C motif) ligand 5 (CCL5) in a dose-and time-dependent manner. Moreover, poly I:C stimulation promoted CCL5-mediated migration in OC2 cells. %26lt;br%26gt;Conclusions: In this report, we provide a novel mechanism for tumor invasion and the TLR3-dependent inflammatory response that could have therapeutic implications for OSCC.

• 出版日期2012-3
• 单位长春大学