A simple agar plate preparation for effective transfer of Ureaplasma colonies onto nitrocellulose membranes, for colony immunoblotting

作者：Zimmerman Carl Ulrich R^*; Stiedl Thomas; Spergser Joachim; Rosengarten Renate

来源：Journal of Microbiological Methods, 2014, 104: 79-81.

DOI：10.1016/j.mimet.2014.06.013

• Ureaplasma
• Colony size
• Colony immunoblot
• Colony morphology
• Multiple banded antigen
• Hemadsorption

A simple method for preparing agar plates is presented, which allows an efficient transfer of Ureaplasma colonies to nitrocellulose membranes for subsequent immunological detection. This simple and reproducible procedure was used to demonstrate antigenic variation in the phase-variable mba-locus of Ureaplasma parvum serovar 3.

• 出版日期2014-9