Development of Sensor Surfaces Using Poly-(N-vinylformamide) for Sensitive Detection of 2,4,6-Trinitrotoluene by Displacement Method on a Surface Plasmon Resonance Sensor

作者：Onodera Takeshi^*; Ishii Ryosuke; Yatabe Rui; Toko Kiyoshi

来源：Sensors and Materials, 2016, 28(3): 209-218.

DOI：10.18494/sam.2016.1258

• surface plasmon resonance
• displacement
• TNT
• poly-N-vinylformamide
• antigen-antibody interaction

To detect 2,4,6-trinitrotoluene (TNT) using a displacement method over a surface plasmon resonance immunosensor, we developed sensor surfaces modified with a poly-(N-vinylformamideco-vinylamine), which was synthesized from a monomer, N-vinylformamide, and was hydrolyzed to produce amine groups, and TNT analogs. Poly-(N-vinylformamide-co-vinylamine) that reacted with succinic acid was used as a matrix of a sensor surface for increasing the number of antibody binding sites. 2,4-Dinitrophenyl glycine, 3-nitrophenyl acetic acid, and 4-nitrophenyl acetic acid were immobilized on the polymer-modified sensor surfaces. We evaluated the suppression ability of the sensor surface for nonspecific adsorption and deteiniined the limit of detection (LOD) for TNT. As a result, a sensor chip suppressed nonspecific adsorption for 1 mg/mL bovine serum albumin and lysozyme. TNT was measured by the displacement method, and the LOD achieved over a 3-nitrophenyl acetic acid-modified sensor chip was 0.9 ng/mL (ppb).

• 出版日期2016