Complete genome sequence of a novel influenza A H1N2 virus circulating in swine from Central Bajio region, Mexico

作者：Sanchez Betancourt J I; Cervantes Torres J B; Saavedra Montanez M; Segura Velazquez R A^*

来源：Transboundary and Emerging Diseases, 2017, 64(6): 2083-2092.

DOI：10.1111/tbed.12620

• genome
• H1N2
• seroprevalence
• swine influenza

The aim of this study was to perform the complete genome sequence of a swine influenza A H1N2 virus strain isolated from a pig in Guanajuato, Mexico (A/swine/Mexico/GtoDMZC01/2014) and to report its seroprevalence in 86 counties at the Central Bajio zone. To understand the evolutionary dynamics of the isolate, we undertook a phylogenetic analysis of the eight gene segments. These data revealed that the isolated virus is a reassortant H1N2 subtype, as its genes are derived from human (HA, NP, PA) and swine (M, NA, PB1, PB2 and NS) influenza viruses. Pig serum samples were analysed by the hemagglutination inhibition test, using wild H1N2 and H3N2 strains (A/swine/Mexico/Mex51/2010 [H3N2]) as antigen sources. Positive samples to the H1N2 subtype were processed using the field-isolated H1N1 subtype (A/swine/Mexico/Ver37/2010 [H1N1]). Seroprevalence to the H1N2 subtype was 26.74% in the sampled counties, being Jalisco the state with highest seroprevalence to this subtype (35.30%). The results herein reported demonstrate that this new, previously unregistered influenza virus subtype in Mexico that shows internal genes from other swine viral subtypes isolated in the past 5years, along with human virus-originated genes, is widely distributed in this area of the country.

• 出版日期2017-12