ACORN BARNACLE MEGABALANUS-ROSA LECTIN (BRA-3) - CDNA CLONING, GENE STRUCTURE AND SEASONAL-CHANGES OF MESSENGER-RNA AND PROTEIN-LEVELS

作者：TAKAMATSU N^*; TAKEDA T; KOJIMA M; HEISHI M; MURAMOTO K; KAMIYA H; SHIBA T

来源：Gene, 1993, 128(2): 251-255.

• CARBOHYDRATE RECOGNITION DOMAIN
• EXON INTRON STRUCTURE
• GENE EXPRESSION
• MARINE INVERTEBRATE
• POLYMERASE CHAIN REACTION
• POLYMORPHISM
• RECOMBINANT DNA

We have isolated cDNA clones coding for a lectin (BRA-3) from the acorn barnacle, Megabalanus rosa. Sequence comparison of the cDNA clones has revealed polymorphism in the BRA-3 mRNA, which results from single-nucleotide (nt) differences at three positions. All three differences are within the coding region and cause conservative amino acid (aa) changes. The BRA-3 gene is composed of four exons, and the three single-nt differences are located on different exons. In addition, the BRA-3 mRNA and BRA-3 protein levels increased during early summer in a similar fashion, indicating that BRA-3 production is regulated mainly at the level of transcription.

• 出版日期1993-6-30