A peptide receptor-based bioelectronic nose for the real-time determination of seafood quality

作者：Lim Jong Hyun; Park Juhun; Ahn Jung Ho; Jin Hye Jun; Hong Seunghun^*; Park Tai Hyun

来源：Biosensors and Bioelectronics, 2013, 39(1): 244-249.

DOI：10.1016/j.bios.2012.07.054

• Trimethylamine
• Carbon nanotube
• Field-effect transistor
• Seafood quality
• Olfactory receptor
• Bioelectronic nose

We herein report a peptide receptor-based bioelectronic nose (PRBN) that can determine the quality of seafood in real-time through measuring the amount of trimethylamine (TMA) generated from spoiled seafood. The PRBN was developed using single walled-carbon nanotube field-effect transistors (SWNT-FETs) functionalized with olfactory receptor-derived peptides (ORPs) which can recognize TMA and it allowed us to sensitively and selectively detect TMA in real-time at concentrations as low as 10 fM. Utilizing these properties, we were able to not only determine the quality of three kinds of seafood (oyster, shrimp, and lobster), but were also able to distinguish spoiled seafood from other types of spoiled foods without any pretreatment processes. Especially, the use of small synthetic peptide rather than the whole protein allowed PRBNs to be simply manufactured through a single-step process and to be reused with high reproducibility due to no requirement of lipid bilayers. Furthermore, the PRBN was produced on a portable scale making it effectively useful for the food industry where the on-site measurement of seafood quality is required.

• 出版日期2013-1-15