ERK MAP-Kinase is Involved in the B7-H1 Expression Induced by Etoposide In Retinoblastoma Cells

作者：Zhou, Haixiao; Liu, Zhigang; Huang, Linying; Yang, Anhuai; Li, Junping; Chen, Zhen^*

来源：Journal of Animal and Veterinary Advances, 2013, 12(3): 369-376.

• Retnoblastoma
• B7-H1
• etoposide
• mitogen-activated protein kinases
• small interfering RNA

The effect of etoposide on B7-H1 expression was determined by the Reverse-Transcription Polymerase Chain Re-action (RT-PCR), real-time PCR and flow cytometry analysis m Y79 cells Then, the involvement of Mitogen-Activated Protein Kinases (MAPKs) signal pathways were tested by Western blotting and signal transduction inhibitor assays Furthmore, specific small interfering RNA (siRNA) targeting B7-H1 was transfected into Y79 retinoblastoma cells using liposome Silencing of B7-H1 expression was measured by RT-PCR and Western blotting assays Etoposide increased B7-H1 mRNA and protein levels m Y79 cells The effect of etoposide on B7-H1 expression peaked at the concentration of 5 mu g mL(-1) as confirmed by RT-PCR, real-time PCR and flow cytometry assays (p<0 01) The phosphoylation status of Extracellular Signal-Regulated Kinase (ERK), c-Jun N-terminal Kinase (JNK) were constitutively activated by etoposide and MEK inhibitor simultaneously reduced the expression of B7-H1 induced by etoposide (p<0 01) B7-H1 siRNA significantly silenced B7-H1 expression in Y79 cells as confirmed by RT-PCR and Western blotting assays (p<0 01)

• 出版日期2013
• 单位武汉大学