The staining pattern of human sperm with Diff Quik: relationship with sperm head morphology and a sperm chromatin structure assay (SCSA)

作者：Moska Natalie^*; Murray Elaine; Wakefield Penelope; Matson Phillip

来源：Reproductive Biology, 2011, 11(1): 55-59.

DOI：10.1016/S1642-431X(12)60064-3

• Diff Quik
• sperm chromatin
• DNA fragmentation index

The dark staining of human sperm heads by Diff Quik is significantly correlated with abnormal sperm head morphology (r=0.51, p < 0.0001), but is not associated with changes in sperm chromatin detected by a sperm chromatin structure assay (SCSA; r=0.18, p > 0.09). Whilst valuable in the assessment of head morphology, it is concluded that Diff Quik staining is not a useful substitute for the SCSA to assess human sperm chromatin. Reproductive Biology 2011 11 1: 55-59.

• 出版日期2011-3