A cryptic plasmid pBL1 from Brevibacterium lactofermentum causes growth inhibition and filamentation in Escherichia coli

作者：Goyal D; Wachi M; Kijima N; Kobayashi M; Yukawa H; Nagai K

来源：Plasmid, 1996, 36(1): 62-66.

DOI：10.1006/plas.1996.0033

A shuttle vector composed of pBL1, a 4.46-kb cryptic plasmid from coryneform bacterium Brevibacterium lactofermentum, and Escherichia coli vector pHSG298 was found to inhibit growth and cause cell filamentation in E. coli. After construction of several deletions of pBL1, a 1.23-kb AccI-HindIII fragment was found responsible. DNA sequence analysis showed that this fragment contained a 726-bp-long open reading frame (ORF3), encoding a protein with 242 amino acid residues. Corresponding to ORF3, a 28-kDa protein was detected in an in vitro protein synthesis system. ORF3 was dispensable for the stable maintenance of pBL1 in coryneform bacteria.

• 出版日期1996-7