摘要

The qualitative and quantitative determination of the chemical constituents in traditional Chinese medicines(TCMs) is an important task, which builds the foundation of the theory of pharmacological activity. The hyphenated chromatographic instruments combined with the related chemometric methods provide powerful tools for the resolution of such complex systems. The volatile chemical constituents of Notoptergium incium (NI) were analyzed by gas chromatography-mass spectrometry (GC-MS) and the overlapped peaks were resolved into pure chromatograms and pure spectra with iterative OPA or non-iterative EWOP chemometrics resolution method. Sixty-five of the 98 separated constituents in essential oil of Notoptergium incium, accounting for 92.13%, were identified and quantified by the developed method.