Analysis of intracellular human immunodeficiency virus (HIV)-1 drug resistance mutations in multi-failed HIV-1-infected patients treated with a salvage regimen: 72-week follow-up

作者：Falasca F; Montagna C; Maida P; Bucci M; Fantauzzi A; Mezzaroma I; Antonelli G^*; Turriziani O

来源：Clinical Microbiology and Infection, 2013, 19(7): E318-E321.

DOI：10.1111/1469-0691.12175

• Archived mutations
• drug resistance
• genotypic analysis
• HIV-1 DNA
• treatment failure

The human immunodeficiency virus (HIV) mutational archive of proviral DNA was monitored during a 72-week follow-up in 20 multidrug-experienced HIV-1-infected patients treated with a darunavir/ritonavir-based salvage therapy. At the beginning of the study, all patients harboured a number of intracellular drug resistance-associated mutations (RAMs) in peripheral blood mononuclear cells. In some patients, a significant fluctuation in the number of RAMs was observed during the observation period. However, all patients, notwithstanding the presence or the fluctuation of intracellular RAMs, showed a persistently undetectable viraemia. The data suggest that the archived resistant viral variants change during suppressive therapy, but that the variants are unable to re-emerge and to affect virological response.

• 出版日期2013-7