A sensitive and specific liquid chromatography-mass spectrometry method for determination of metacavir in rat plasma

作者：Li Zhan; Huang Xin; Jiang Zhenzhou; Xiao Yajie; Liu Chunhui; Zhang Luyong^*; Shu Bin; Huang Jingfeng; Li Tao; Wang Tao; Wang Feng

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2008, 864(1-2): 9-14.

DOI：10.1016/j.jchromb.2007.12.022

• liquid chromatography/mass spectrometry
• HBV
• metacavir
• rat plasma
• pharmacokinetics

A sensitive and specific liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method has been developed and validated for the quantification of metacavir in rat plasma using tinidazole as an internal standard (I.S.). Following ethyl acetate extraction, the analytes were separated on a Shim-pack ODS (4.6 mu m, 150 mm x 2.0 mm I.D.) column and analyzed in selected ion monitoring (SIM) mode with a positive ESI interface using the respective [M + H](+) ions, 266 for metacavir and 248 for tinidazole. The method was validated over the concentration range of 1-600 ng/mL for metacavir. Between and within-batch precisions (R.S.D.%) were all within 15% and accuracy (%) ranged from 92.2 to 105.8%. The lower limit of quantification (LLOQ) was 1 ng/mL. The extraction recovery was on average 89.8%. The validated method was used for the pharmacokinetic study of metacavir in rats.

• 出版日期2008-3-15
• 单位中国药科大学