A disulfide linkage in a CCCH zinc finger motif of an Arabidopsis CPSF30 ortholog

作者：Addepalli Balasubrahmanyam^*; Limbach Patrick A; Hunt Arthur G

来源：FEBS Letters, 2010, 584(21): 4408-4412.

DOI：10.1016/j.febslet.2010.09.043

• CPSF30
• CCCH zinc finger
• Endonuclease
• Disulfide linkage
• Mass spectrometry

The Arabidopsis ortholog of the 30 kDa subunit of the cleavage and polyadenylation factor (AtCPSF30) is an RNA binding endonuclease, and the endonuclease activity is inhibited by reducing agents. Here, we report the presence of a disulfide linkage in the endonuclease motif based on comparative mass spectrometry (MS) analysis of reduced and non-reduced but carbamidomethylated protein. This analysis reveals that this disulfide bond involves a CCCH zinc finger motif, one that is associated with the endonuclease activity of AtCPSF30. This finding raises the possibility that redox regulation of AtCPSF30 may occur through oxidation and reduction of the disulfide linkage.

• 出版日期2010-11-5