An Experimental Model for Induction of Lung Cancer in Rats by Chlamydia Pneumoniae

作者：Chu, De-Jie^*; Guo, Shui-Gen; Pan, Chun-Feng; Wang, Jing; Du, Yong; Lu, Xu-Feng; Yu, Zhu-Yuan

来源：Asian Pacific Journal of Cancer Prevention, 2012, 13(6): 2819-2822.

DOI：10.7314/APJCP.2012.13.6.2819

• Chlamydia pneumonia
• benzo (a) pyrene
• lung cancer
• animal model
• rat

Objective: To assess induction effects of Chlamydia pneumoniae (Cpn) on lung cancer in rats. Methods: A lung cancer animal model was developed through repeated intratracheal injection of Cpn (TW-183) into the lungs of rats, with or without exposure to benzo(a) pyrene (Bp). Cpn antibodies (Cpn-IgA, -IgG, and -IgM) in serum were measured by microimmunofluorescence. Cpn-DNA or Cpn-Ag of rat lung cancer was detected through polymerase chain reaction or enzyme-linked immunosorbent assay. Results: The prevalence of Cpn infection was 72.9% (35/48) in the Cpn group and 76.7% (33/43) in the Cpn plus benzo(a) pyrene (Bp) group, with incidences of lung carcinomas in the two groups of 14.6% (7/48) and 44.2% (19/43), respectively (P-values 0.001 and <0.000 compared with normal controls). Conclusions: A rat model of lung carcinoma induced by Cpn infection was successfully established in the laboratory for future studies on the treatment, prevention, and mechanisms of the disease.

• 出版日期2012
• 单位复旦大学