Analysis of Microbial Population Dynamics in a Partial Nitrifying SBR at Ambient Temperature

作者：Zeng, Taotao; Li, Dong; Zeng, Huiping; Zhang, Zhao; Liu, Liqian; Zhang, Xiaojing; Zhang, Jie^*

来源：Current Microbiology, 2013, 66(6): 614-620.

DOI：10.1007/s00284-013-0317-6

• OXIDIZING BACTERIAL COMMUNITIES
• GRADIENT GEL-ELECTROPHORESIS
• 16S RIBOSOMAL-RNA
• PARTIAL NITRIFICATION
• DISSOLVED-OXYGEN
• AMMONIA
• QUANTIFICATION
• NITRITATION
• PERFORMANCE
• DIVERSITY

In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 A degrees C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study microbial population dynamics. Partial nitrification was effectively achieved in response to shifts of influent ammonium concentrations. DGGE results showed that higher ammonia concentration referred to lower ammonia-oxidizing bacteria (AOB) diversity in the SBR. Phylogenetic analysis revealed that all the predominant AOB was affiliated with Nitrosomonas genus. FISH analysis illustrated AOB was the predominant nitrifying bacteria of microbial compositions when SBR achieved partial nitrification (PN) at ambient temperature.

• 出版日期2013-6
• 单位哈尔滨工业大学; 北京工业大学