Molecular and histological identification of the acanthocephalan Bolbosoma cf. capitatum from the human small intestine

作者：Arizono Naoki^*; Kuramochi Toshiaki; Kagei Noboru

来源：Parasitology International, 2012, 61(4): 715-718.

DOI：10.1016/j.parint.2012.05.011

• Acanthocephala
• Bolbosoma capitatum
• Bolbosoma nipponicum
• Corynosoma
• Human infection
• Molecular identification

Acanthocephalans of the genus Bolbosoma are intestinal parasites of marine mammals with a lifecycle similar to that of anisakid nematodes. Several cases of Bolbosoma infection in humans have been reported, but no species identification has been made. Here, we report a case of Bolbosoma infection, in which the worm was found in histological sections of the partially resected small intestine of a Japanese man. Morphological features of the worm reconstructed from serial sectioning indicated that the worm was most likely to be a sexually immature female of Bolbosoma capitatum. DNA extraction from paraffin-embedded sections and ITS1-5.8S rRNA-ITS2 sequencing showed that this species formed a monophyletic group with Bolbosoma nipponicum, and was clearly distinguishable from Corynosoma spp. or Polymorphus spp. These results may provide a reference for identifying and characterizing unknown acanthocephalans found in histological sections.

• 出版日期2012-12