A novel dendrimeric peptide induces high level neutralizing antibodies against classical swine fever virus in rabbits

作者：Li, Guo-Xin; Zhou, Yan-Jun; Yu, Hai; Li, Ling; Wang, Ya-Xin; Tong, Wu; Hou, Jun-Wei; Xu, Yan-Zhao; Zhu, Jian-Ping; Xu, Ao-Tian; Tong, Guang-Zhi^*

来源：Veterinary Microbiology, 2012, 156(1-2): 200-204.

DOI：10.1016/j.vetmic.2011.10.012

• Classical swine fever virus
• Dendrimeric peptide
• Neutralizing epitope
• T helper cell epitope
• Marker vaccine

The amino acid sequence (TAVSPTTLR, 829-837 aa) on the glycoprotein E2 of classical swine fever virus (CSFV) is a conserved and linear neutralizing epitope. In the present study, two peptides were constructed based the core sequence of this neutralizing epitope, the dendrimeric peptide (Th-B-4) containing four copies of B cell epitope fused to one copy of promiscuous T helper (Th) cell epitope and the peptide Th-B containing a single copy of B cell epitope fused to one copy of Th cell epitope. The dendrimeric peptide Th-B-4 elicited high titers of neutralizing antibodies as detected in an indirect ELISA, blocking ELISA and neutralization test and induced a complete protection against CSFV C strain in rabbits. The Th-B elicited low titers of neutralizing antibodies and did not induce a protection in rabbits. These results suggest that the dendrimeric peptide Th-B-4 may be a promising marker vaccine candidate against CSFV and the multimerization is a requirement for development of a peptide vaccine.

• 出版日期2012-4-23
• 单位中国农业科学院; 中国农业科学院上海兽医研究所