A Rat Model of Studying Tissue-Type Plasminogen Activator Thrombolysis in Ischemic Stroke With Diabetes

作者：Fan Xiang; Qiu Jianhua; Yu Zhanyang; Dai Haibin; Singhal Aneesh B; Lo Eng H; Wang Xiaoying^*

来源：Stroke, 2012, 43(2): 567-570.

DOI：10.1161/STROKEAHA.111.635250

• diabetes
• rats
• thrombolytics
• tPA

Background and Purpose-Poststroke hyperglycemia and diabetes mellitus are associated with lower thrombolytic efficacy and an increased risk of postischemic cerebral hemorrhage. We aimed to develop a rodent model of thrombolysis in diabetic stroke that mimics the clinical situation.
Method-Male 6-week Type I diabetic rats (14 weeks old) were subjected to embolic focal stroke and treated with tissue-type plasminogen activator at 1.5 hours. Reperfusion and 24-hour neurological outcomes were measured and compared with nondiabetic control rats.
Results-Diabetic rats exhibited resistance to thrombolytic reperfusion, larger infarction volumes, and increased intracerebral hemorrhage.
Conclusions-This animal model would be relevant to future studies investigating pathophysiological mechanisms and in developing new therapeutic approaches to enhance the efficacy of tissue-type plasminogen activator thrombolysis in stroke patients with diabetes or poststroke hyperglycemia. (Stroke. 2012;43:567-570.)

• 出版日期2012-2