Analysis of bisphenols in soft drinks by on-line solid phase extraction fast liquid chromatography tandem mass spectrometry

作者：Gallart Ayala H; Moyano E^*; Galceran M T

来源：Analytica Chimica Acta, 2011, 683(2): 227-233.

DOI：10.1016/j.aca.2010.10.034

• Bisphenols
• Soft drink beverages
• Fused-Core (TM) column
• On-line solid phase extraction (SPE)

In this study, an automated on-line solid-phase extraction coupled to fast liquid chromatography-tandem mass spectrometry (on-line SPE fast LC-MS/MS) method was developed for the simultaneous analysis of bisphenol A (BPA), bisphenol F (BPF), bisphenol E (BPE), bisphenol B (BPB) and bisphenol S (BPS) in canned soft drinks without any previous sample treatment. A C18 (12 mu m particle size) loading column was used for the SPE on-line preconcentration before the liquid chromatography baseline separation of bisphenol compounds using a C18 Fused-Core (TM) (50 mm x 2.1 mm i.d.) column, which took less than 3 min. Gradient elution and heated electrospray were used to reduce matrix effect and improve ionization efficiency. To select the most intense and selective transitions, fragmentation studies were performed by multiple-stage mass spectrometry in an ion trap mass analyzer and tandem mass spectrometry in a triple quadrupole instrument, this latter instrument being used for quantitation in SRM mode. Quality parameters of the method were established and we obtained a simple, fast, reproducible (RSD values lower than 10%) and accurate (precision higher than 93%) method for the analysis of bisphenols in canned soft drinks at the ng L(-1) level using matrix-matched calibration.

• 出版日期2011-1-10