A Simple, Rapid, Efficient and Inexpensive Strategy for Sequencing Clones from cDNA Libraries

作者：Nguyen Dinh Truong^*; Oh Younshin; Dirisala Vijaya R; Choi Hojun; Park Keun Kyu; Kim Jin Hoi; Park Chankyu

来源：Biotechnology and Bioprocess Engineering, 2010, 15(5): 817-821.

DOI：10.1007/s12257-009-3039-7

• cDNA library
• screening
• sequencing
• colony PCR

In this report, we describe a simple, rapid, efficient and inexpensive strategy for sequencing inserted DNAs from clones of cDNA or gDNA libraries. This strategy uses PCR products directly amplified from transformed bacterial colonies, with universal primers within the vector. The method can be applied for sequencing cDNA or gDNA libraries with up to 4 similar to 5 kb insert sizes, without overnight liquid culture or plasmid DNA preparation steps. We successfully used this method to analyze clones from full-length, enriched cDNA libraries. Although simple, following this strategy will significantly help researchers to avoid unnecessary steps in the analysis of a cDNA library.

• 出版日期2010-10