A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase

作者：Gao, Gang; Wang, An; Gong, Bo-Liang; Li, Qing-Qing; Liu, Yu-Huan; He, Zhu-Mei^*; Li, Gang^*

来源：Enzyme and Microbial Technology, 2016, 84: 24-31.

DOI：10.1016/j.enzmictec.2015.12.005

• Coptotermes formosanus
• Metagenomic library
• Phosphotransferase system
• Glucosidase
• Glucose tolerance

Functional screening of a metagenomic library of termite hindgut identified an overlapping gene cluster encoding the phosphotransferase system (PTS) components, which consisted of a glucoside specific PTS enzyme II gene (glu1923) and a glycoside hydrolase gene (glu1392). Hydrolytic experiments revealed that the combined effect of Glul 923 and Glu1392 was responsible for the utilization of glucosidic substrates in recombinant Escherichia coli (E. coli) strains. Yeast two hybrid analysis suggested that there was an interaction between Glu1923 and Glu1392, and the domain EIIA of Glu1923 played an important role for the interaction. In addition, the hydrolase Glu1392 displayed hydrolysis ability toward cellobiose and maltose, and had a very high tolerance to glucose with a K-i value of 2.25 M. These properties make Glu1392 an interesting candidate in biotechnology applications after further study.

• 出版日期2016-3
• 单位中山大学