Assembly and Calcium Binding Properties of Quantum Dot-Calmodulin Calcium Sensor

作者：Eun Su Yong; Kim Nguyen Ta; Yoo Hoon; Silva Gabriel A^*; Kim Soon Jong^*

来源：Journal of Nanoscience and Nanotechnology, 2016, 16(2): 2065-2068.

DOI：10.1166/jnn.2016.11921

• Calcium Sensor
• Quantum Dot
• Calmodulin
• FRET
• Stoichiometry
• Ratiometric
• Dissociation Constant

We have developed the first nanoengineered quantum dot molecular complex designed to measure changes of calcium ion (Ca2+) concentration at high spatial and temporal resolutions in real time. The sensor is ratiometric and composed of three components: a quantum dot (QD) emitting at 620 nm as a fluorescence donor, an organic dye (Alexa Fluor 647) as a fluorescence acceptor, and a calmodulin-M13 (CaM-M13) protein part as a calcium sensing component. In this work, we have determined the maximal number of CaM-M13 required for saturating a single QD particle to be approximately 16. The dissociation constant, K-d of the QD-based calcium ion sensor was also estimated to be around 30 mu M.

• 出版日期2016-2