A noninvasive method for measuring the oxygen binding-releasing capacity of hemoglobin-loaded polymeric nanoparticles as oxygen carrier

作者：Zhang, Xiaolan^*; Liu, Changsheng; Yuan, Yuan; Shan, Xiaoqian; Sheng, Yan; Xu, Feng

来源：Journal of Materials Science: Materials in Medicine , 2009, 20(5): 1025-1030.

DOI：10.1007/s10856-008-3676-y

• BOVINE HEMOGLOBIN
• BLOOD SUBSTITUTES
• ARTIFICIAL BLOOD
• PH
• PARAMETERS

Based on the strong penetration capacity of near infrared lights (NIRs) and different absorption of oxyhemoglobin and deoxyhemoglobin in NIRs region, a novel noninvasive method, with the aid of an airproof-equilibrium apparatus, was developed to determine the oxygen binding-releasing capacity, including oxygen dissociation curve (ODC) and P-50, of the hemoglobin-loaded polymeric nanoparticles (HbP) in this study. The measured ODC of the PLA-PEG HbP was very close to that of the native hemoglobin, and the corresponding P-50 (26.1 mmHg) was also near to the native precursor protein (27.3 mmHg), indicative of the validity of the method proposed. To further verify the method proposed, the oxygen binding-releasing capacity of the HbPs prepared by PCL, PCL-PEG, PLA were also investigated with human blood as control. These results indicated that the method developed here enabled accurate and noninvasive determination of the oxygen binding-releasing capacity of the biodegradable polymeric oxygen carriers.

• 出版日期2009-5
• 单位华东理工大学