Ultrasensitive detection of carcinoembryonic antigen based on electrochemiluminescence quenching of Ru(bpy)(3)(2+) by quantum dots

作者：Hao Tingting; Guo Zhiyong^*; Du Shuping; Shi Lili

来源：Sensors and Actuators B: Chemical , 2012, 171: 803-809.

DOI：10.1016/j.snb.2012.05.074

• Electrochemiluminescence immunosensor
• Carcinoembryonic antigen
• Ru(bpy)(3)(2+)-graphene-Nafion composites
• ECL quenching

An ultrasensitive electrochemiluminescence (ECL) immunosensor for the detection of carcinoembryonic antigen (CEA) in serum and saliva was developed. Ru(bpy)(3)(2+)-graphene-Nafion composite was first coated on the glassy carbon electrode surface in this ECL immunosensor. The sandwich-type immunoreactions between the first antibody and the second antibody bridged the donors Ru(bpy)(3)(2+) and acceptors QDs, which led to the occurrence of ECL quenching of Ru(bpy)(3)(2+) by QDs. Based on the efficient ECL quenching, ultrasensitive CEA detection was realized. Under optimal conditions, the ECL signal depended linearly on the logarithm of the CEA concentration within a range from 0.005 to 0.5 pg/mL, and the detection limit of CEA was 0.002 pg/mL. Moreover, the proposed ECL immunosensor provides a stable, specific, and highly sensitive immunoassay protocol for CEA detection at very low levels, which might hold a promise for clinical application.

• 出版日期2012-9
• 单位宁波大学