Embryonic Expression of the Chicken Kruppel-Like (KLF) Transcription Factor Gene Family

作者：Antin Parker B^*; Pier Maricela; Sesepasara Terry; Yatskievych Tatiana A; Darnell Diana K

来源：Developmental Dynamics, 2010, 239(6): 1879-1887.

DOI：10.1002/dvdy.22318

• chicken embryo
• in situ hybridization
• Kruppel-like factor
• KLF

The Kruppel-like transcription factors (KLF) are zinc finger proteins that activate and suppress target gene transcription. Although KLF factors have been implicated in regulating many developmental processes, a comprehensive gene expression analysis has not been reported. Here we present the chicken KLF gene family and expression during the first five days of embryonic development. Fourteen chicken KLF genes or expressed sequences have been previously identified. Through synteny analysis and cDNA mapping, we have identified the KLF9 gene and determined that the gene presently named KLF1 is the true ortholog of KLF17 in other species. In situ hybridization expression analyses show that in general KLFs are broadly expressed in multiple cell and tissue types. Expression of KLFs 3, 7, 8, and 9, is widespread at all stages examined. KLFs 2, 4, 5, 6, 10, 11, 15, and 17 show more restricted patterns that suggest multiple functions during early stages of embryonic development. Developmental Dynamics 239:1879 1887, 2010.

• 出版日期2010-6