Anaerobic degradation of benzene by enriched consortia with humic acids as terminal electron acceptors

作者：Cervantes Francisco J^*; Mancilla Ana Rosa; Emilia Rios del Toro E; Alpuche Solis Angel G; Montoya Lorenzana Lilia

来源：Journal of Hazardous Materials, 2011, 195: 201-207.

DOI：10.1016/j.jhazmat.2011.08.028

• Anaerobic benzene oxidation
• Hydrocarbon biodegradation
• Humus respiration
• Quinones

The anaerobic degradation of benzene coupled to the reduction of humic acids (HA) was demonstrated in two enriched consortia. Both inocula were able to oxidize benzene under strict anaerobic conditions when the humic model compound, anthraquinone-2,6-disulfonate (AQDS), was supplied as terminal electron acceptor. An enrichment culture originated from a contaminated soil was also able to oxidize benzene linked to the reduction of highly purified soil humic acids (HPSHA). In HPSHA-amended cultures, 9.3 mu M of benzene were degraded, which corresponds to 279 +/- 27 micro-electron equivalents (mu Eq) L-1, linked to the reduction of 619 +/- 81 mu Eq L-1 of HPSHA. Neither anaerobic benzene oxidation nor reduction of HPSHA occurred in sterilized controls. Anaerobic benzene oxidation did not occur in soil incubations lacking HPSHA. Furthermore, negligible reduction of HPSHA occurred in the absence of benzene. The enrichment culture derived from this soil was dominated by two gamma-Proteobacteria phylotypes. A benzene-degrading AQDS-reducing enrichment originated from a sediment sample showed the prevalence of different species from classes beta-, delta- and gamma-Proteobacteria. The present study provides clear quantitative demonstration of anaerobic degradation of benzene coupled to the reduction of HA.

• 出版日期2011-11-15