A GC/MS method for the simultaneous determination and quantification of chlorpromazine and diazepam in pork samples

作者：Zhang Liqun; Wu Pinggu^*; Zhang Yiming; Jin Quan; Yang Dajin; Wang Liyuan; Zhang Jing

来源：Analytical Methods, 2014, 6(2): 503-508.

DOI：10.1039/c3ay41130c

A gas chromatography coupled with mass spectrometry (GC/MS) method for the simultaneous determination of chlorpromazine and diazepam in pork samples, which are two of the most often used antipsychotics, has been developed and validated. The samples were prepared and purified by solid-phase extraction (SPE), and then were analyzed by GC/MS. Chromatography was performed using an HP-5 MS 5% phenyl-95% methylpolysiloxane fused silica capillary column (30 m x 0.25 mu m; film thickness 0.25 mm). The method had a linear calibration curve over the range 10-500 ng mL(-1) (r > 0.99), recovery (>66.5% for chlorpromazine and >77.4% for diazepam), precision (%RSD < 11.5% for chlorpromazine and <9.5% for diazepam). The limit of quantification for both chlorpromazine and diazepam was 2.0 mu g kg(-1). This method can result in faster analysis, lower solvent consumption and less workload per sample while maintaining or even improving sensitivity.

• 出版日期2014
• 单位中国疾病预防控制中心; 浙江省疾病预防控制中心; 杭州市疾病预防控制中心