Association of hsp70-2 (+1267A/G), hsp70-hom (+2437T/C), HMOX-1 (number of GT repeats) and TNF-alpha (+489G/A) polymorphisms with COPD in Croatian population

作者：Matokanovic Mirela^*; Rumora Lada; Popovic Grle Sanja; Cepelak Ivana; Culic Ognjen; Barisic Karmela

来源：Clinical Biochemistry, 2012, 45(10-11): 770-774.

DOI：10.1016/j.clinbiochem.2012.04.003

• TNF-alpha
• HMOX-1
• hsp70-hom
• hsp70-2
• COPD
• Croatian population
• SNP
• Genetic susceptibility

Objective: To test for possible association of hsp70-2 (+1267A/G), hsp70-hom (+2437T/C), HMOX-1 (number of GT repeats) and TNF-alpha (+489G/A) polymorphisms with chronic obstructive pulmonary disease (COPD) in Croatian population. %26lt;br%26gt;Methods: Genotyping of DNA isolated from whole blood of 130 COPD patients (as defined by spirometry) and 95 healthy controls was performed. Fragment size analysis upon restriction enzyme digestion and/or sequencing was used for genotype/allele definition. Significance of findings was tested using chi(2) test. %26lt;br%26gt;Results: hsp70-2 (+1267A/G) polymorphism was significantly associated with COPD. Results of genotyping analysis indicated that a genotype carrying G allele was preferentially associated with COPD; odds ratio (OR) = 1.50, 95% confidence interval (CI)= 1.00-2.24 and P=0.061. OR for the GG genotype was 3.47 with Cl = 1.26-9.56 and P=0.04. No association for hsp70-hom (+2437T/C), TNF-alpha (+489G/A) and HMOX-1 (number of GT repeats) polymorphisms were found. In addition, comparison of genotype frequencies among different stages of disease severity (GOLD II-IV) revealed no discrimination for any of the tested polymorphisms. %26lt;br%26gt;Conclusion: This study is supporting the association of hsp70-2 (+1267A/G) polymorphism and COPD. Higher frequency of G allele and GG genotype in Croatian COPD patients was observed. There was no evidence for the association of hsp70-hom (+2437T/C), TNF-alpha (+489G/A) SNPs and HMOX-1 (number of GT repeats) polymorphism with COPD. Allele and genotype frequencies for all of the tested polymorphisms show no association with disease severity (GOLD II-IV).

• 出版日期2012-7