UCA1, a long noncoding RNA, promotes the proliferation of CRC cells via p53/p21 signaling

作者：Shen Yi; Ying Xiao-jiang; Li Zhen-jun; Li Gang; Xue Wu-jin; Jie Wei-xia^*

来源：Open Life Sciences, 2016, 11(1): 206-210.

DOI：10.1515/biol-2016-0027

• long non-coding RNA
• UCA1
• CRC
• p53/p21 signaling

Objective: Recently, the role of long non-coding RNAs (lncRNAs) in human colorectal cancer (CRC) has been a subject of intense focus. We set out to determine the function of one lncRNA, termed urothelial carcinoma-associated 1 (UCA1) in CRC cell proliferation and its underlying mechanisms. Methods: Quantitative real-time PCR (qRT-PCR) was applied to detect the expression level of UCA1 in 50 pairs of CRC samples compared with non-tumor colon tissues. Cell growth was determined using the Cell Counting Kit-8 (CCK-8). Western blotting was carried out to analyze the related protein expression. Flow cytometry was done to evaluate cell apoptosis by UCA1 inhibition. Results: We found an increased expression of UCA1 in CRC samples. Knockdown of UCA1 in HCT116 cells induced a decrease in cell proliferation rate compared to control samples. This oncogenic activity may be enhanced through p53/p21 signaling. Conclusion: Our results supported the hypothesis that upregulation of UCA1 contributes to the unlimited proliferation rate of CRC cells, at least partially through the negative regulation of p53/p21 signaling pathway. Finally, we found that UCA1 merely influenced CRC cell apoptosis.

• 出版日期2016-1
• 单位浙江大学; 绍兴市人民医院