A Functional Role for CCR6 on Proallergic T Cells in the Gastrointestinal Tract

作者：Blazquez Ana Belen; Knight Adina Kay; Getachew Hoheteberhan; Bromberg Jonathan S; Lira Sergio A; Mayer Lloyd; Berin M Cecilia^*

来源：Gastroenterology, 2010, 138(1): 275-284.

DOI：10.1053/j.gastro.2009.09.016

BACKGROUND & AIMS: CCL20 is a chemokine that regulates the homeostatic and inflammatory trafficking of leukocytes to the small intestine and regulates the development OF the gastrointestinal lymphoid architecture. T cells expressing T helper cell (Th)2 cytokines are critical for experimental food allergy, and we hypothesized that CCL20 is involved in the localization of these cells to the gut. METHODS: We evaluated the role of CCR6 in allergic diarrhea induced by sensitization and oral challenge with ovalbumin (OVA) using CCR6(+/+) and CCR6(-/-) mice. RESULTS: CCR6(-/-) mice were protected from OVA-induced diarrhea but surprisingly were not impaired in mastocytosis or allergen-specific immunoglobulin E. CCR6(-/-) mice were also protected from T cell-mediated diarrhea induced by anti-CD3 antibody. Allergic diarrhea was associated with an increased expression of Th2 cytokines within the intestinal mucosa that was significantly reduced in CCR6(-/-) mice. Inhibition OF lymphocyte homing by treatment with FTY720 did not impair allergic diarrhea, indicating that reactivation of T cells Could Occur locally within the small intestine. Finally, T-cell transfer studies demonstrated that CCR6 was required both on the transferred T cells and in the recipient Mouse to manifest allergic disease in the gastrointestinal tract. CONCLUSIONS: These studies highlight a mast cell- and immunoglobulin E-independent role for CCR6-bearing T cells in the pathogenesis of gastrointestinal allergic disease.

• 出版日期2010-1