A live Salmonella enterica serovar enteritidis vaccine allows serological differentiation between vaccinated and infected animals

作者：Adriaensen Connie; De Greve Henri; Tian Jean Q; De Craeye Stphane; Gubbels Eline; Eeckhaut Venessa; Van Immerseel Filip; Ducatelle Richard; Kumar Mahesh; Hernalsteens Jean Pierre^*

来源：Infection and Immunity, 2007, 75(5): 2461-2468.

DOI：10.1128/IAI.01357-06

Three precisely defined deletion mutants of Salmonella enterica serovar Enteritidis were constructed, a guanine auxotrophic Delta guaB mutant, a nonflagellated Delta fliC mutant, and an auxotrophic and nonflagellated Delta guaB Delta fliC double mutant. All three mutants were less invasive than the wild-type strain in primary chicken cecal epithelial cells and the human epithelial cell line T84 and less efficiently internalized in the chicken macrophage cell line HD11. The Delta fliC mutant was pathogenic in orally infected BALB/c mice, while the Delta guaB mutant was attenuated and conferred protection against a challenge with the pathogenic parent strain. The Delta guaB Delta fliC double mutant was totally asymptomatic and conferred better protection than the Delta guaB mutant. This indicates that the major flagellar protein flagellin is not required for efficient vaccination of BALB/c mice against Salmonella infection. The Delta guaB Delta fliC mutant was also safe for vaccination of 1-day-old chickens. After two immunizations, it induced statistically significant protection against infection of the internal organs of the birds by a virulent S. enterica serovar Enteritidis challenge strain but not against intestinal colonization. These data demonstrate that nonflagellated attenuated Salmonella mutants can be used as marker vaccines.

• 出版日期2007-5