A MUTATION AT THE UNIVERSALLY CONSERVED POSITION-529 IN ESCHERICHIA-COLI 16S RIBOSOMAL-RNA CREATES A FUNCTIONAL BUT HIGHLY ERROR-PRONE RIBOSOME

作者：SANTER UV; CEKLENIAK J; KANSIL S; SANTER M; OCONNOR M; DAHLBERG AE

来源：RNA-A Publication of the RNA Society, 1995, 1(1): 89-94.

• A SITE
• RIBOSOMAL-RNA MUTANT
• 30S SUBUNIT
• TRANSLATIONAL FIDELITY

A base substitution of G to U was constructed at position 529 in Escherichia coli 16S rRNA. The U529 mutant ribosomes were functional and present on polysomes but were highly error prone and caused a progressive loss of cell viability. They displayed elevated levels of readthrough of stop codons and frameshifting, and an increase in thermal sensitivity of beta-galactosidase, suggestive of missense errors. These results demonstrate that the universally conserved G529 is involved in tRNA selection at the A site during protein synthesis.

• 出版日期1995-3