Discovery and mapping of a new expressed sequence tag-single nucleotide polymorphism and simple sequence repeat panel for large-scale genetic studies and breeding of Theobroma cacao L.

作者：Allegre Mathilde; Argout Xavier^*; Boccara Michel; Fouet Olivier; Roguet Yolande; Berard Aurelie; Thevenin Jean Marc; Chauveau Aurelie; Rivallan Ronan; Clement Didier; Courtois Brigitte; Gramacho Karina; Boland Auge Anne; Tahi Mathias; Umaharan Pathmanathan; Brunel Dominique; Lanaud Claire

来源：DNA Research, 2012, 19(1): 23-35.

DOI：10.1093/dnares/dsr039

• Theobroma cacao
• genetic map
• SNP
• molecular marker

Theobroma cacao is an economically important tree of several tropical countries. Its genetic improvement is essential to provide protection against major diseases and improve chocolate quality. We discovered and mapped new expressed sequence tag-single nucleotide polymorphism (EST-SNP) and simple sequence repeat (SSR) markers and constructed a high-density genetic map. By screening 149 650 ESTs, 5246 SNPs were detected in silico, of which 1536 corresponded to genes with a putative function, while 851 had a clear polymorphic pattern across a collection of genetic resources. In addition, 409 new SSR markers were detected on the Criollo genome. Lastly, 681 new EST-SNPs and 163 new SSRs were added to the pre-existing 418 co-dominant markers to construct a large consensus genetic map. This high-density map and the set of new genetic markers identified in this study are a milestone in cocoa genomics and for marker-assisted breeding. The data are available at http://tropgenedb.cirad.fr.

• 出版日期2012-2
• 单位中国地震局