Mechanisms associated with cellular desiccation tolerance of Artemia encysted embryos from locations around the world

作者：Hengherr Steffen^*; Schill Ralph O; Clegg J S

来源：Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology, 2011, 160(2): 137-142.

DOI：10.1016/j.cbpa.2011.05.032

• Artemia cysts
• DSC
• Glass transition temperature
• Biological glasses
• Stress proteins

Using differential scanning calorimetry we demonstrated the presence of biological glasses and measured the glass transition temperatures (Tg) in dry encysted gastrula embryos (cysts) of the brine shrimp, Artemia, from eleven different locations, two of which provided cysts from parthenogenetic animals. Values for Tg were highest, by far, in Anemia franciscana cysts from the Mekong Delta, Vietnam (VN), these cysts having been produced from previous sequential inoculations into growth ponds of cysts from the San Francisco Bay, California, USA. Tg values for three groups of A. franciscana cysts were significantly higher than those of other cysts (except those of Artemia persimilis) studied here, as well as all other desiccation-tolerant animal systems studied to date. We also measured three stress proteins (hsc70, artemin and p26) in all these cysts as well as the total alcohol soluble carbohydrates (ASC), about 90% of which is the disaccharide trehalose, a known component of biological glasses. We i

• 出版日期2011-10