Associations between Genetic Polymorphisms in IL-33, IL1R1 and Risk for Inflammatory Bowel Disease

作者：Latiano Anna^*; Palmieri Orazio; Pastorelli Luca; Vecchi Maurizio; Pizarro Theresa T; Bossa Fabrizio; Merla Giuseppe; Augello Bartolomeo; Latiano Tiziana; Corritore Giuseppe; Settesoldi Alessia; Valvano Maria Rosa; D' Inca Renata; Stronati Laura; Annese Vito; Andriulli Angelo

来源：PLos One, 2013, 8(4): e62144.

DOI：10.1371/journal.pone.0062144

Background: Recent evidence suggests that the IL-33/IL1RL1 axis plays a critical role in several autoimmune and inflammatory disorders; however, its mechanistic role in inflammatory bowel disease (IBD) has not been clearly defined. We investigated the contribution of IL-33 and IL1RL1 polymorphisms to IBD risk, and possible correlations with phenotype in an Italian cohort of adult and pediatric patients. %26lt;br%26gt;Methods: We evaluated the association of six SNPs in IL-33 and IL1RL1 genes, in 805 Crohn%26apos;s disease (CD), 816 ulcerative colitis (UC), and 752 controls, using Taqman. IL-33 and IL1RL1 mRNA expression was also analyzed. %26lt;br%26gt;Results: Significant allele and genotype associations with IL-33 rs3939286 were found in CD (P = 0.004; P = 0.035) and UC patients (P = 0.002; P = 0.038). After stratifying the cohort for age at diagnosis, the differences remained significant only in the IBD adult-onset. Significant associations were also obtained in CD patients with two IL1RL1 polymorphisms (rs13015714 and rs2058660, P %26lt; 0.015). By combining homo-and heterozygous carriers of the rs13015714 risk allele, differences were still significant for both CD adult-and pediatric-onset. Upon genotype-phenotype evaluation, an increased frequency of extensive colitis in adult UC (P = 0.019) and in steroid-responsive pediatric patients (P = 0.024) carrying the IL-33 rs3939286 risk genotype, was observed. mRNA expression of IL-33 and IL1RL1 in inflamed IBD biopsy samples was significantly increased. %26lt;br%26gt;Conclusions: Common IL-33 and IL1RL1 polymorphisms contribute to the risk of IBD in an Italian cohort of adult and pediatric patients, with some influence on sub-phenotypes.

• 出版日期2013-4-25