A three-dimensional model of primary bovine endometrium using an electrospun scaffold

作者：MacKintosh S B^*; Serino L P; Iddon P D; Brown R; Conlan R S; Wright C J; Maffeis T G G; Raxworthy M J; Sheldon I M

来源：Biofabrication, 2015, 7(2): 025010.

DOI：10.1088/1758-5090/7/2/025010

• scaffold
• co-culture
• endometrium
• polyglycolide
• uterus
• SEM
• in vitro

Endometrial stromal and epithelial cell function is typically studied in vitro using standard two-dimensional monocultures, but these cultures fail to reflect the complex three-dimensional (3D) architecture of tissue. A 3D model of bovine endometrium that reflects the architectural arrangement of in vivo tissue would beneficially assist the study of tissue function. An electrospun polyglycolide (PGA) scaffold was selected to grow a 3D model of primary bovine endometrial epithelial and stromal cells, that reflects the architecture of the endometrium for the study of pathophysiology. Electrospun scaffolds were seeded with stromal and epithelial cells, and growth was assessed using histological techniques. Prostaglandin E-2 and prostaglandin F-2 alpha responsiveness of endometrial scaffold constructs was tested using oxytocin plus arachidonic acid (OT + AA) or lipopolysaccharide (LPS). Stromal and epithelial cells growing on the electrospun scaffold had an architectural arrangement that mimicked whole tissue, deposited fibronectin, had appropriate expression of vimentin and cytokeratin and were responsive to OT + AA and LPS, as measured by prostaglandin accumulation. In conclusion, a functional 3D model of stromal and epithelial cells was developed using a PGA electrospun scaffold which may be used to study endometrial pathophysiology.

• 出版日期2015-6