Application of green fluorescent protein to affinity electrophoresis; Affinity of IgG-binding domain C from streptococcal protein G to mouse IgG1

作者：Kazama H^*; Yamada K; Aoki T; Watabe H

来源：Biological & Pharmaceutical Bulletin, 2002, 25(2): 168-171.

DOI：10.1248/bpb.25.168

• affinity electrophoresis
• green fluorescent protein
• streptococcal protein G
• immunoglobulin G

Affinity electrophoresis (AEP) using green fluorescent protein (GFP) was studied. We constructed a fusion protein that linked S147PGFP and IgG binding domain C from streptococcal protein G (GFP-SpGC). The affinity of GFP-SpGC for mouse IgG1 was measured. The AEP using GFP does not require a staining step after electrophoresis. and was successful with a non-purified sample. Therefore, this method is simple and useful for measuring many samples such as those used in mutational studies.

• 出版日期2002-2