ASSOCIATION OF CLASS-II SEQUENCES ENCODING DR1 AND DQ5 SPECIFICITIES WITH HYPERSENSITIVITY TO CHIRONOMID ALLERGEN CHI-T-I

作者：TAUTZ C; RIHS HP; THIELE A; ZWOLLO P; FREIDHOFF LR; MARSH DG; BAUR X

来源：Journal of Allergy and Clinical Immunology, 1994, 93(5): 918-925.

DOI：10.1016/0091-6749(94)90386-7

• ALLERGY
• HLA-D TYPING
• POLYMERASE CHAIN REACTION
• SEQUENCE-SPECIFIC OLIGONUCLEOTIDES

A panel of 188 unrelated Caucasian subjects who were exposed to the larvae of Chironomus thummi (Diptera, nonbiting midges) was HLA-typed by polymerase chain reaction amplification of the second exons of the DRB, DQA1, and DQB1 genes followed by dot-blot hybridization with sequence-specific oligonucleotide probes. Type I sensitization to the allergen Chi t I and a large number of other inhalant allergens was determined by RAST and skin testing. Sixty-one individuals were found to be sensitized to Chi t I, of whom 24 were sensitive to this allergen and to no other allergens tested. Statistical analyses showed that only in the latter group were the HLA-D genes DRB1*0101, DQA1*0101, and DQB1*0501 associated with IgE-responsiveness to Chi t I. These results suggest that HLA associations responsiveness to certain allergens may be more striking in monosensitized subjects.

• 出版日期1994-5