Allelic loss in amalgam-associated oral lichenoid lesions compared to oral lichen planus and mucosa

作者：Rodrigues L N; Sousa S F; Silva R C C; Abreu M H G; Pires F R; Mesquita R A; Bastos Rodrigues L; De Marco L; Gomes C C; Gomez R S; Bernardes V F^*

来源：Oral Diseases, 2017, 23(4): 471-476.

DOI：10.1111/odi.12627

• amalgam-associated oral lichenoid lesion
• oral lichen planus
• loss of heterozygosity
• cell proliferation index

BackgroundThe amalgam-associated oral lichenoid lesion (AAOLL) shows clinical and histopathological features similar to oral lichen planus (OLP). Molecular researches to improve knowledge of pathogenesis and clinical behavior of AAOLL are still scarce.
ObjectiveWe investigated for the first time the use of loss of heterozygosity (LOH) as a molecular approach for genetic characterization of AAOLL in comparison with OLP and evaluated the cell proliferation index.
Materials and MethodsThe sample comprised nine AAOLLs, 10 OLPs, and eight NOMs matched by patients' gender and age. LOH was assessed using polymorphic microsatellite markers at chromosomes 9p (D9S157, D9S162, D9S171), 11q (D11S1369), and 17p (TP53, AFM238WF2). Cell proliferation was assessed by immunohistochemical expression of Ki-67 (MIB-1). The association between LOH and Ki-67 was investigated.
ResultsLoss of heterozygosity occurred in 5/9 AAOLLs and in 2/10 OLPs in at least one marker each, while NOM showed no LOH. Cell proliferation index in AAOLL ranged from 2 to 23%. There was no association between cell proliferation and LOH, independent of the marker.
ConclusionOur study shows that the profile of molecular changes in AAOLL and OLP, evaluated by LOH and Ki-67 expression, is similar. Additional studies including larger samples should be performed to confirm or to refute our findings.

• 出版日期2017-5