Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini- and Conventional STR Kits

作者：Oh Chang Seok; Lee Sang Jun; Park Jun Bum; Lee Soong Deok; Seo Seung Bum; Kim Hye Yeon; Kim Jaehyup; Kim Yi Suk; Shin Dong Hoon^*

来源：Journal of Forensic Sciences, 2012, 57(3): 820-825.

DOI：10.1111/j.1556-4029.2011.02044.x

• forensic science
• autosomal short tandem repeat genotyping
• medieval human sample
• AmpFlSTR (R) MiniFiler (TM) kit
• AmpFlSTR (R) Identifiler (TM) kit
• ancient DNA
• Korea

Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFlSTR (R) MiniFiler (TM) kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFlSTR (R) MiniFiler (TM) and Identifiler (TM) kits. Whereas only 21.87% of larger-sized loci profiles could be obtained with the Identifiler (TM) kit, 75% of the same loci profiles were determined by MiniFiler (TM) kit analysis. This very successful amplification of large-sized STR markers from highly degraded aDNA suggests that the MiniFiler (TM) kit could be a useful complement to conventional STR kit analysis of ancient samples.

• 出版日期2012-5