Direct Membrane Association Drives Mitochondrial Fission by the Parkinson Disease-associated Protein alpha-Synuclein

作者：Nakamura Ken; Nemani Venu M; Azarbal Farnaz; Skibinski Gaia; Levy Jon M; Egami Kiyoshi; Munishkina Larissa; Zhang Jue; Gardner Brooke; Wakabayashi Junko; Sesaki Hiromi; Cheng Yifan; Finkbeiner Steven; Nus**aum Robert L; Masliah Eliezer; Edwards Robert H^*

来源：Journal of Biological Chemistry, 2011, 286(23): 20710-20726.

DOI：10.1074/jbc.M110.213538

The protein alpha-synuclein has a central role in Parkinson disease, but the mechanism by which it contributes to neural degeneration remains unknown. We now show that the expression of alpha-synuclein in mammalian cells, including neurons in vitro and in vivo, causes the fragmentation of mitochondria. The effect is specific for synuclein, with more fragmentation by alpha- than beta- or gamma-isoforms, and it is not accompanied by changes in the morphology of other organelles or in mitochondrial membrane potential. However, mitochondrial fragmentation is eventually followed by a decline in respiration and neuronal death. The fragmentation does not require the mitochondrial fission protein Drp1 and involves a direct interaction of synuclein with mitochondrial membranes. In vitro, synuclein fragments artificial membranes containing the mitochondrial lipid cardiolipin, and this effect is specific for the small oligomeric forms of synuclein. alpha-Synuclein thus exerts a primary and direct effect on the morphology of an organelle long implicated in the pathogenesis of Parkinson disease.

• 出版日期2011-6-10