A Comparative Study on Different Characteristics between Prostate Cancer Model and BPH Model of BALB/c Mice

作者：Sun Weigui^*; Gan Yiping; Ye Zhangqun; Yu Qiangguo; Mi Zhenguo; Song Xiaosong; Fan Zhaoyin; Wang Quanhong; Han Chunzhi

来源：Gene Therapy and Molecular Biology, 2009, 13A: 205-213.

• Mouse
• Model
• Method
• Prostate Cancer
• PIN
• BPH
• Characteristics

Objective: To comparatively study the different characteristics between spontaneous PCa (prostate cancer) and BPH (benign prostatic hyperplasia) of mice, we improved methods to simultaneously build their models. Methods: 75 BALB/c male mice were randomly divided into 5 groups: the control mice (A), the castrated mice (B), the castrated mice with T (testosterone) treatment (C), the castrated mice with MNU (N-methyl-N-nitrosourea) treatment (D), and the castrated mice with both T and MNU treatments (E). As to T treatment, 12.5mg.kg(-1) T solution was injected into a mouse's abdominal cavity once every two days. As regards MNU treatment, 100mg.kg(-1) MNU solution was injected into a mouse's prostate once every two weeks. The whole experiment was finished in the ninth weekend and all mice were sacrificed. Their blood-samples were collected from eyeballs for the purpose of serous proteins, cytokines and sexual hormones analyses, while their prostates were studied on anatomy and pathology. Results: The prostates in-group A were normal whereas those in group B appeared atrophy. The group C showed BPH model which had the highest serous T and T/E(2) (testosterone / estradiol) (P>0.05). The group D supplied us with mild PIN (prostatic intraepithelial neoplasia) model of 40% incidence, while the group E provided us with severe PIN and early cancers of 92.86% incidence, which had the highest serous VEGF (vascular endothelial growth factor) (P>0.05). In addition, groups CDE had apparently higher serous T and T/E(2) than groups AB (P<0.05), however, serous E(2) had no distinct difference among 5 groups (P>0.05). With regard to serous proteins analyses by SELDI-TOF-MS as contrasted with BPH model, 12 abnormal proteins in-group E had been discovered which probably have a lot to do with cancerous. Conclusion: Homeochronous models of prostatic BPH, PIN and early cancer could have been quickly obtained in 9 weeks, which would be possibly helpful for us to comparatively study on their mechanisms, characteristics, relationships and differential diagnoses in future.

• 出版日期2009
• 单位华中科技大学