Decolourisation of anthraquinone- and anthracene-type dyes by versatile peroxidases from bjerkandera fumosa and pleurotus ostreatus D1

作者：Pozdnyakova Natalia N^*; Jarosz Wilkolazka Anna; Polak Jolanta; Graz Marcin; Turkovskaya Olga V

来源：Biocatalysis and Biotransformation, 2015, 33(2): 69-80.

DOI：10.3109/10242422.2015.1060227

• Anthracene- and anthraquinone-type dyes
• decolourisation
• fungal versatile peroxidases

The catalytic properties of a versatile peroxidase from Pleurotus ostreatus D1 (Jacquin) P. Kummer were studied in comparison with that of a typical versatile peroxidase from Bjerkandera fumosa 137 (Per.:Fr) Karst. Decolourisation activities of both enzymes towards a wide range of dyes containing condensed aromatic rings (anthraquinone-and anthracene-type) were found. The anthraquinone dyes were decolourised rapidly by both tested peroxidases. The presence of polymerisation reaction products of Acid Blue 62, Basic Blue 22 and Reactive Blue 4 oxidation, and breakdown of aromatic rings of Alizarin Red were observed. The main catalytic constants (K-M and V-max) of the decolourisation reactions of anthraquinone dyes were calculated. In the case of Alizarin Red, inhibition of the activity of versatile peroxidase from P. ostreatus D1 by an excess of the substrate was observed. Independence from Mn2+ ions of the catalytic activity of versatile peroxidase from P. ostreatus D1 towards different substrates was revealed. Finally, differences in the catalytic activity towards anthracene-type dyes and monoaromatic substrates of both peroxidases were found.

• 出版日期2015-4