科研之友
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摘要
Zebrafish is one of the most used vertebrate model organisms in molecular and developmental biology, recently gaining popularity also in medical research However, very little work has been done to assess zebrafish as a model species in nutritional studies in aquaculture in order to utilize the methodological toolbox that this species represents As a starting point to acquire some baseline data for further nutritional studies, growth of a population of zebrafish was followed for 15 weeks Furthermore, whole body proteome was screened during development by means of bi-dimensional gel electrophoresis and mass spectrometry Fish were reared under best practice laboratory conditions from hatching until 103 days post-fertilization (dpf) and regularly fed ad libitum with Anemia nauplii from 12 dpf A growth burst occurred within 9-51 dpf, reaching a plateau after 65 dpf Fork length and body weight were significantly lower in males than in females from 58 dpf onwards Proteorims analysis showed 28 spot proteins differently expressed through development and according to sex Of these proteins, 20 were successfully identified revealing proteins involved in energy production, muscle development, eye lens differentiation, and sexual maturation In summary, zebrafish exhibited a rapid growth until approximately 50 dpf, when most individuals started to allocate part of the dietary energy intake for sexual maturation However, proteomic analysis revealed that some individuals reached sexual maturity earlier and already from 30 dpf onwards Thus, in order to design nutritional studies with zebrafish fed Anemia nauplii, it is recommended to select a period between 20 and 40 dpf, when fish allocate most of the Ingested energy for non-reproductive growth purposes
- 出版日期2010-12
